Anne Mari A. Rokstad
93084332
424.04.029 420 Kunnskapssenteret Øya, Trondheim
Forskning
Et hovedfokus i forskningen vår er å forstå hvordan immunforsvaret reagerer på materialer som oppfattes som fremmede. Slike materialer kan komme utenfra kroppen, slik som mikro‑ og nanoplast fra miljøet eller ulike mikro‑ og nanomaterialer brukt i medisinsk behandling. De kan også være utviklet for terapeutisk bruk, slik som biomaterialer som brukes til å kapsle inn celler. I tillegg kan kroppen selv danne strukturer som fungerer som «fremmedstoffer», for eksempel kolesterolkrystaller som oppstår ved kardiovaskulær sykdom. Felles for alle disse materialene er at de kan aktivere immunforsvaret og utløse betennelsesprosesser, men de gjør det gjennom både overlappende og spesifikke mekanismer.
I den pågående forskningen ligger hovedvekten på mikro‑ og nanoplast, hvor vi undersøker hvordan materialegenskaper, slik som størrelse, form og overflate, påvirker aktivering av immun‑ og betennelsesresponser i blod. Formålet er å forstå hva denne typen miljøeksponering kan bety for menneskers helse, og om enkelte typer plastpartikler gir særlig sterke eller uheldige immunologiske effekter.
Som en egen, men beslektet forskningslinje studerer vi endogene materialer som kolesterolkrystaller. Disse dannes inne i kroppen og kan være kraftige triggere for inflammasjon, spesielt i utviklingen av aterosklerose og andre hjerte‑ og karsykdommer. Ved å sammenligne immunaktivering fra kolesterolkrystaller med reaksjoner indusert av miljøpartikler som mikroplast, får vi innsikt i hvilke mekanismer som er universelle og hvilke som er spesifikke for ulike typer materialer.
I et separat forskningsspor arbeider vi med biomaterialer til medisinsk bruk, særlig materialer som brukes ved innkapsling av celler til avanserte terapier. Her undersøker vi hvordan design og egenskaper ved slike materialer påvirker immunrespons, med mål om å utvikle tryggere og mer biokompatible løsninger, blant annet for bruk i behandling av type 1‑diabetes.
På tvers av disse prosjektene er målet å forstå hvordan immunsystemet responderer på ulike typer materialer, og hvordan denne kunnskapen kan brukes til både å bedre folkehelsen og å utvikle mer presise og sikre medisinske behandlinger.
Publikasjoner
Type 1 diabetes mellitus (T1DM) does not affect whole blood responses to alginate-based microspheres despite plasma lipid and glucose differences
First study to investigate initial inflammatory capacity between T1DM and controls towards biomaterials, bioparticles and TLR ligands. We find that these responses are similar despite identifying metabolic differences in lipoproteins and metabolites.
MS-proteomics provides insight into the host responses towards alginate microspheres
First study to map host proteins of various alginate microspheres of relevance in cell therapy. Study find differences to inflammatory pathways. Fibrosis free microspheres bind naturally and enrich for anti-complement and anti-coagulation proteins.
Cholesterol crystals induce coagulation activation through complement-dependent expression of monocytic tissue factor
Study showing a link between cholesterol crystals and coagulation activation. Cholesterol crystals found in atherosclerotic plaques could be contributors in thrombosis.
Formidling
2025
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Konferanseforedrag(2025) Generalities on MNPs and human health. Micro- and Nanoplastics: Workplace Exposure and Potential Health Hazards
2024
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Intervju
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Intervju
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Konferanseforedrag
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Konferanseposter
2023
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Foredrag(2023) MS-proteomics provides insight into the host responses towards alginate microspheres. A HEALTHY FUTURE
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Konferanseposter(2023) Understanding the Human Health Impacts of Micro- and nanoplastics. Microplastics - Workshop for early career reaseachers
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Konferanseposter
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Konferanseposter(2023) The initial host response to alginate-based microbeads in human whole blood depict by functional response and mass spectrometry. ScSB meeting - Bioinspiration in biomaterials
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Konferanseposter
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Konferanseposter(2023) Characterizing the Inflammatory Properties of Novel Fucoidan/Alginate Beads. ScSB meeting - Bioinspiration in biomaterials
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Konferanseforedrag
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Konferanseposter
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Konferanseposter(2023) MS-based proteomic profiling of alginate microspheres relevant for cell therapy. ScSB meeting - Bioinspiration in biomaterials
2019
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Konferanseposter(2019) Elucidating host responses towards alginate microspheres using proteomics. Scandinavian Society for Biomaterials 2019 Conference
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Konferanseposter(2019) Cholesterol crystals induce thromboinflammation. Conference on molecular mechanisms in Trondheim
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Konferanseposter(2019) Multicomponent polyelectrolyte complex-based microcapsules: Correlation between the encapsulation conditions and microcapsule properties. 83rd PRAGUE MEETING ON MACROMOLECULES
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Intervju
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Intervju
2018
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Konferanseposter(2018) Identification of adsorbing human plasma proteins on alginate gel beads. BRG Capsule workshop 2018
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Konferanseposter(2018) Identification of adsorbing human plasma proteins on alginate gel beads. Nordic Proteomics Conference
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Konferanseposter
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Konferanseforedrag(2018) Unmasking the inflammatory properties of a material by the human whole blood model. Teveltunet Conference 2018
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Foredrag(2018) Celle-innkapslings teknologi ved bruk av alginat – konsept, utfordringer og forskning. Tekna Biotek Trondheim Høstseminar
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Konferanseforedrag(2018) Understanding biomaterial inflammation through the whole blood model. 10th Training School on Microencapsulation
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Konferanseforedrag(2018) Cholesterol crystals induce coagulation that can be prevented by targeting complement. 11th International Conference on Complement Therapeutics
2017
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Foredrag
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Konferanseforedrag(2017) The update on multicomponent PMCG microcapsule and on related activities. JDRF Consortium monthly call
2016
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Foredrag(2016) Multicomponent microcapsules for allogeneic islet transplantation in a comprehensive, preclinical non-human primate model. Encapsulation Consortium Spring Meeting
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Foredrag(2016) Multicomponent microcapsules for islets transplantation - tuning their properties towards a low-inflammatory surface by assessments in a human whole blood model. 2nd International Conference on Immune Responses to Biosurfaces: Mechanisms and Therapeutic Interventions
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Konferanseposter(2016) Cholesterol crystals induce coagulation activation in a complement-dependent manner. Conference on Molecular Mechanisms of Inflammation
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Konferanseposter(2016) Effect of rapid weight loss in obese individuals with focus on inflammatory receptor expression in peripheral blood leukocytes. Conference on Molecular Mechanisms of Inflammation
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Konferanseforedrag(2016) Effekt av en VLCD diett på markører for inflammasjon, metabolisme og hjerte kar risisko. Fedmeforskningsdagene 2016 i Trondheim
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Konferanseposter(2016) Cholesterol crystals induce coagulation activation in a complement-dependent manner. 2nd International Conference on Immune Responses to Biosurfaces: Mechanisms and Therapeutic Interventions
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Foredrag(2016) Atherosclerosis coagulation and obesity inflammation – is complement the answer?. The annual CEMIR seminar
2015
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Konferanseposter(2015) Crystalline cholesterol is found in atherosclerotic plaques and induces complement mediated inflammation. 15th European Meeting on Complement in Human Disease
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Konferanseforedrag
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Konferanseforedrag
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Foredrag(2015) Multicomponent microcapsules for allogeneic islet transplantation in a comprehensive, preclinical non-human primate model. , Encapsulation consortium JDRF meeting (presented by Igor Lacik)
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Foredrag(2015) Multicomponent microcapsules for allogeneic islet transplantation in a comprehensive, preclinical non-human primate model. (presented by Jose Oberholzer). Encapsulation consortium JDRF meeting
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Foredrag(2015) THE COMPLEMENT C3 DEPOSITION DEPENDS ON THE ALGINATE MICROSPHERES COMPOSITION AND DETERMINDS THE CYTOKINE PROFILE. Functional polymers at bio-material interfaces, 79th Prague meeting on macromolecules
2014
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Foredrag(2014) Inflammatory Reactions to Microcapsules in a Human Whole Blood Model. Instituttseminar Inst bioteknologi (NTNU)
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Foredrag(2014) Inflammatory Reactions to Microcapsules in a Human Whole Blood Model. Tissue Engineering of the Bioartificial Pancreas to Functionally Cure Diabetes
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Konferanseforedrag(2014) Wood nanocellulose – assessment of toxicity and effects on activation of the complement and coagulation pathways using a whole blood model. Cellulose nanotechnology research
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Konferanseforedrag
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Foredrag(2014) Inflammatory and hemostatic activation induced by alginate microcapsules reveals novel mechanisms underlying their biocompatibility. Immune responces to biosurfaces: Mechanisms and therapeutic interventions
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Foredrag(2014) Initial inflammatory and haemostatic mechanisms induced by endogenous and bioartificial surfaces in a whole blood model. NBS contact Meeting 2014
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Konferanseforedrag
2012
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Konferanseforedrag
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Konferanseposter(2012) Poly-cation containing alginate microcapsules induce cytokines by a complement-dependent mechanism. XXIV. International Complement Workshop
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Konferanseforedrag
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Foredrag
2011
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Konferanseforedrag(2011) Alginate Microcapsules for the Treatment of Type 1 Diabetes. National Congress of the Norwegian Chemical Society, Invited speaker
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Konferanseforedrag(2011) Inflammatory responses of alginate microspehers evaluated by a human and baboon whole blood model. 13th world Congress of IPITA International Pancreas and Islets Transplant Association
2008
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Konferanseforedrag(2008) Alginate capsules and biocompatibility issues. Tirsdagsmøte for DMF, NTNU og St. Olavs Hospital
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Konferanseposter(2008) TOLL like receptor 4 is involved in inflammatory reactions (overgrowth) against alginate capsules. TOLL2008 Meeting; Recent advances in Pattern recognition
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Konferanseforedrag
2007
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Intervju(2007) "Håp for kreft".
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Konferanseforedrag(2007) Kan TOLL-reseptorer og adaptormolekyler være involvert i overvekst på alginatkapsler?. TOLL-gruppe møte
2006
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Foredrag
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Konferanseforedrag(2006) Cell-compatible covalent alginate beads obtained from a chemoenzymatically engineered alginate. XIV International workshop on bioencapsulation & COST 865 meeting
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Konferanseforedrag
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Konferanseforedrag
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Konferanseforedrag
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Konferanseforedrag
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Konferanseforedrag(2006) Improvement of Alginate Microbeads using enzymatic modification. XIVth International Workshop on Bioencapsulation
2005
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Konferanseposter(2005) Making alginate bioreactors for treatment of brain tumours: strategies for optimalisation of the system. The Norwegian Biochemical Society 41st Contact Meeting
2002
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Konferanseforedrag
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Konferanseforedrag(2002) Evaluation of different alginate microcapsules as bioreactors for 293-EBNA cells delivering the anti-angiogenic factor endostatin. 38.Biokjemiske kontaktmøte Functional genomics-the mosaics of life
2001
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Foredrag
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Konferanseposter(2001) Immunostimulation as counteraction to bacterial problems in first feeding of marine larve. European Aquaculture Society Meeting
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Konferanseposter(2001) Epimerised alginate: effect on biocompatibility of microcapsules. Scandinavian Socisty for the Study of Diabetes
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Konferanseforedrag(2001) Improved capsule properties by the use of epimerised alginate. The Ninth International Workshop in Bioencapsulation
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Konferanseforedrag(2001) Improved biocompatibility of alginate-Poly-L-lysine-alginate microcapsules prepared with epimerized alginates. The 8th World Congress of the International Pancreas & Islet Transplant Association
2000
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Konferanseposter(2000) Transplantation of Alginate Microcapsules with proliferating cells in mice: Capsular overgrowth and survival of encapsulated cells in allo-and xeno graft modells. Bioartificial organs III. Tissue Sourcing, Immunosolation and Clinical Trials.
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Konferanseposter(2000) Identification of cells in the fibrotic reaction against transplanted encapsulated cells. Islets in Eilat: A combined islet basic science and transplantation meeting
1999
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Foredrag(1999) In vitro og in vivo effekter av alginater på piggvarleukocytter. Fiske- og dyrehelsemøtet 1999
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Foredrag(1999) Alginates as adjuvant in fish vaccine against Aeromonas salmonicida. 35. Biokjemiske Kontaktmøte, NBS