Bakgrunn og aktiviteter

Fagfelt /arbeidsområde

Jeg jobber som førsteamanuensis II / forsker ved Inst. for bioteknologi. Mitt arbeidsområder er innen transkripsjonell og translasjonell regulering av bakteriell genekspresjon og syntetisk biologi. Modellorganismer som vi jobber med er: Escherichia coli, Pseudomonas putida KT2440, psychrophilic Pseudomonas, Psychrobacter spp., Corynebacterium glutamicum, Synechococcus sp. PCC 7002, og Saccharomyces cerevisiae.

Projekter

EU - H2020 - Advanced toolbox for rapid and cost-effective functional metagenomic screening - microbiology meets microfluidics. NTNU Prosjektleder

PhotoSynLab - Vi praktiserer åpen vitenskap

For de som er interessert, sjekk her for å se hva vi gjør: www.photosynlab.org

Undervisning

TBT4165 - System Biology and Biological Networks
TBT4505 - Biotechnology -  Specialization Course - Bioinformatics

Vitenskapelig profil

Scopus
Google Scholar
PubMed
ResearchGate
ORCID

Publikasjoner

  • 2017

Ramisetty SK, Langlette P, Lale R, Dias RdS. In vitro studies of DNA condensation by bridging protein in a crowding environment. International Journal of Biological Macromolecules.

Vogel AIM, Lale R, Hohmann-Marriott MF. Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in Synechococcus sp. PCC 7002. Journal of Biological Engineering.

Lewin A, Lale R, Wentzel A. Expression Platforms for Functional Metagenomics – Emerging technology options beyond Escherichia coli. In Press

  • 2016

Beal J, Haddock-Angelli T, Gershater M, de Mora K, Lizarazo M, Hollenhorst J, et al. Reproducibility of fluorescent expression from engineered biological constructs in E. coli. PLoS ONE.

Arnfinnsdottir, NB., Bjørkøy, A., Lale, R., Sletmoen, M. Heterogeneity in GFP expression in isogenic populations of P. putida KT2440 investigated using flow cytometry and bacterial microarrays. RSC Advances

Moghadam MM, Albersmeier A,Winkler A, Cimmino L, Rise K, Hohmann-Marriott FM, Kalinowski J, Rückert C, Wentzel A, Lale, R. Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity. BMC Genomics.

  • 2015

Bjerga, GE, Lale, R., Williamson, A. Engineering low-temperature expression systems for heterologous expression of cold-adapted enzymes. Bioengineered

Arnfinnsdottir, NB., Ottesen, V., Lale, R., Sletmoen, M. The design of simple bacterial microarrays. Development towards immobilizing single living bacteria on predefined micro-sized spots on patterned surfaces. PLoS ONE.

  • 2014

Petrov, V., Balasubramaniam, S., Lale, R., Moltchanov, D., Lio’, P., Koucheryavy, Y. Forward and Reverse Coding for Chromosome Transfer in Bacterial Nanonetworks. Nano Communication Networks.

Strand, TA., Lale, R., Degnes, KF., Lando, M. and Valla, S. A new and improved host-independent plasmid system for RK2-based conjugal transfer. PLoS ONE.

DNA Cloning and Assembly Methods. Methods in Molecular Biology, editors Rahmi Lale and Svein Valla. Volume 1116, XI, 303 p. Springer Protocols, Humana Press.

Røkke, G., Korvald, E., Pahr, J., Øyås, O., and Lale, R. BioBrick assembly standards and techniques and associated software tools. DNA Cloning and Assembly Methods. Methods in Molecular Biology, editors Lale, R. and Valla, S. Volume 1116, 2014, pp 1-24 Springer Protocols, Humana Press.

  • 2013

Zwick, F., Lale, R. and Valla, S. Regulation of the expression level of transcription factor XylS reveals new functional insight into its induction mechanism at the Pm promoter. BMC Microbiol. 13: 262

Zwick, F., Lale, R. and Valla, S. Combinatorial engineering for heterologous gene expression. Bioengineered.

Balzer, S., Kucharova, V., Megerle, J., Lale, R., Brautaset, T., Valla, S. A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli. Microbial Cell Factories, 12:26.

  • 2012

Zwick, F., Lale, R. and Valla, S. Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory expression control elements in the expression cassette. Microbial Cell Factories, 11:133.

Almaas, E., Bruheim, P., Lale, R. and Valla, S. Dynamics and robustness of metabolic networks: A systems biology review of Escherichia coli metabolism. Systems Microbiology: Current Topics and Applications. ISBN: 978-1-908230-02-7.

  • 2011

Aakvik, T., Lale, R., Liles, M. and Valla, S. Metagenomic libraries for functional screening, Ch 22. Handbook of Molecular Microbial Ecology I: Metagenomics and Complementary Approaches. Ed. de Bruijn, F.J.

Lale, R., Brautaset, T.and Valla, S. Broad-host-range plasmid vectors for gene expression in bacteria. Met. Mol. Biol. 765, Part 3, 327-343.

Lale, R., Berg, L., Stüttgen, F., Netzer, R., Stafsnes, M., Brautaset, T., Aune, T.E.V. and Valla. S. Continuous control of the flow in biochemical pathways through 5'-UTR sequence modifications in mRNA expressed from the broad-host-range Pm promoter. Appl. Env. Mircobiol. 77: 2648-2655.

  • 2010

Aune, T. E. V., Bakke, I., Drabløs, F., Lale, R., Brautaset, T. and Valla, S. Directed evolution of the transcription factor XylS for development of improved expression systems. Microb. Biotechnol. 3:1, 38–47.

  • 2009

Berg, L., Lale, R., Bakke, I., Burroughs, N. and Valla, S. The expression of recombinant genes in Escherichia coli can be strongly stimulated at the transcript production level by mutating the DNA-region corresponding to the 5'-untranslated part of mRNA. Microb. Biotechnol. 2(3), 379-389.

Brautaset, T., Lale, R. and Valla, S. Positively regulated bacterial expression systems. Microb. Biotechnol. 2 (1), 15-30.

  • 2008

Bar, N. S. and Lale, R. Modeling and control of the protein synthesis process in eukaryotic cells. 47th IEEE Proceedings: Conference on Decision and Control (CDC) 179-184.

  • 2007

Lale, R., Tukel, C. and Akcelik, M. Protein profile and plasmid content of Lactococcus lactis subsp lactis LL5Z and Lactococcus lactis subsp cremoris LC79 strains under several stress conditions. T. J. Vet. An. Sci. 31(4), 247-252.