Mirta Mittelstedt Leal de Sousa

Forsker Institutt for klinisk og molekylær medisin
Erling Skjalgssons g 1, Laboratoriesenteret * 231.05.019

Bakgrunn og aktiviteter

Research interests:

  • Proteomics
  • Cancer Research
  • DNA Repair
  • Biochemistry

Formal Education:

PhD in Molecular Medicine, 2012.
Department of Cancer Research and Molecular Medicine, NTNU, Norway.

MSc in Molecular Biology, 2003.
Department of Cellular Biology Brasília, University of Brasília, Brazil.

BSc in Chemistry, 2000.
Department of Chemistry Brasília, University of Brasília, Brazil.

Postdoctoral Institutions

Department of Cancer Research and Molecular Medicine, NTNU, Norway.

Lawrence Berkeley National Laboratory (LBNL), Berkeley, California, USA.
The Joint Bioenergy Institute (JBEI), Emeryville, California, USA.

List of publications:

23. p38 MAPK signaling and phosphorylations in the BRCT1 domain regulate XRCC1 recruitment to sites of DNA damage. Sousa M.M.L., Bjørås K.Ø., Hanssen-Bauer, A., Solvang-Garten, K., Otterlei M. Nature Scientific Reports. Ahead of print (2017). DOI: 10.1038/s41598-017-06770-3.

22. Monitoring of the spatial and temporal dynamics of BER/SSBR pathway proteins, including MYH, UNG2, MPG, NTH1 and NEIL1-3, during DNA replication. Bjørås K.Ø., Sousa M.M.L., Sharma A., Fonseca D.M., Søgaard C.K., Bjørås M., Otterlei M. Nuclei Acids Research. Ahead of print (2017). DOI: 10.1093/nar/gkx476.

21. Proteome alterations associated with transformation of multiple myeloma to secondary plasma cell leukemia. Zatula A., Dikic A., Mulder C., Sharma A., Vågbø C.B., Sousa M.M.L., Waage A., Slupphaug G. Oncotarget. 8(12):19427-19442 (2017). DOI: 10.18632/oncotarget.14294.

20. Novel UCHL1 mutations reveal new insights into ubiquitin processingNovel UCHL1 mutations reveal new insights into ubiquitin processing. Rydning S.L., Backe P.H., Sousa M.M.L., Iqbal Z., Øye A.M., Sheng Y., Yang M., Lin X., Slupphaug G., Nordenmark T.H., Vigeland M.D., Bjørås M., Tallaksen C.M., Selmer K.K. Hum Mol Genet. 26 (6): 1031-1040 (2017). DOI: 10.1093/hmg/ddw391.

19. Analysis of the biotechnological potential of a Lentinus crinitus isolate in the light of its secretome. Cambri G., Sousa M.M.L., Fonseca D.M., Marchini F., Silveira J.L.M., Paba J. Journal of Proteome Research 15(12):4557-4568 (2016). DOI: 10.1021/acs.jproteome.6b00636.

18. Enhanced base excision repair capacity in carotid atherosclerosis may protect nuclear DNA but not mitochondrial DNA. Skarpegland T., Dahl T.B., Skjelland M., Scheffler K., Sousa M.M.L., Gregersen I., Kusnierczyk A., Sharma A., Slupphaug G., Eide L., Segers F.M., Skagen K.R., Dahl C.P., Russell D., Folkersen L., Krohg-Sørensen K., Holm S., Bjørås M., Aukrust P., Halvorsen B. Free Radic Biol Med. 97:386-397 (2016). DOI: http://doi.org/10.1016/j.freeradbiomed.2016.07.001.

17. Aerobic interval training reduces inducible ventricular arrhytmias in diabetic mice after myocardial infarction. Rolim N.P., Skårdal K., Høydal M., Sousa M.M.L., Malmo V., Kaurstad G., Ingul C.B., Hansen H.E., Alves M.N., Thuen M., Haraldseth O., Brum P.C., Slupphaug G., Loennechen J.P., Stølen T., Wisløff U. Basic Res Cardiol 110(4):44 (2015). DOI:10.1007/s00395-015-0502-9.

16. Modulation of cell metabolic pathways and oxidative stress signaling contribute to acquired melphalan resistance in multiple myeloma cells. Zub K.A., Sousa M.M.L., Sarno A., Sharma A., Demirovic A., Rao S., Young C., Aas P.A., Ericsson I., Sundan A., Jensen O.N., Slupphaug G. PlosOne. Mar 13;10(3):e0119857 (2015). DOI: 10.1371/journal.pone.0119857.

15. AID expression in B-cell lymphomas causes accumulation of genomic uracil and a distinct AID mutational signature. Pettersen, H.S., Galashevskaya, A., Doseth, B., Sousa, M.M.L., Sarno, A., Visnes, T., Aas, P.A., Liabakk, N.B., Slupphaug, G., Sætrom, P., Kavli, B.M., Krokan, H.E. DNA repair. 25:60-71 (2015). DOI:10.1016/j.dnarep.2014.11.006.

14. A kinetic-based approach to understanding heterologous mevalonate pathway function in E. coli. Weaver, L.J., Sousa, M.M.L., Wang, G., Baidoo, E., Petzold, C.J., Keasling, J.D. Biotechnology and Bioengeneering. 112(1):111-9 (2015). DOI: 10.1002/bit.25323.

13. A targeted proteomics toolkit for high-throughput absolute quantification of Escherichia coli proteins. Batth, T.S., Singh, P., Ramakrishnan, V.R., Sousa, M.M.L., Chan, L.J.G., Tran, H.M. Luning, E.G., Pan, E.H.Y., Vuu, K.M., Keasling, J.D., Adams, P.D., Petzold, C.J. Metabolic Engeneering. 26C:48-56 (2014). DOI: 10.1016/j.ymben.2014.08.004.

12. Atrial myocyte function and Ca2+ handling is associated with inborn aerobic capacity. Johnsen, A.B., Rolim, N.P.L., Stølen, T., Alves, M., Sousa, M.M.L., Slupphaug, G., Britton, S.L., Koch, L.G., Smith, G.L., Wisløff, U., Høydal, M.A. Plos One. 8(10):e76568 (2013).

11. An inverse switch in DNA base excision and strand break repair contributes to Melphalan resistance in multiple myeloma cells. Sousa, M.M.L., Zub, K.A., Aas, P.A., Hanssen-Bauer, A., Demirovic, A., Sarno, A., Tian, E., Liabakk, N.B., Slupphaug, G. Plos One. 8(2):e55493 (2013).

10. Eating behavior and glucagon-like peptide-1 producing cells in interposed ileum and pancreatic islets in rats subjected to ileal interposition associated with sleeve gastrectomy. Johannessen, H., Kodama, Y., Zhao, C., Sousa, M.M.L, Slupphaug, G., Kulseng, B.E., Chen, D. Obesity Surgery. 23(1):39-49 (2013).

9. Antibody cross-linking and target elution protocols used for immunoprecipitation significantly modulate signal-to-noise ratio in downstream 2D-PAGE analysis. Sousa, M.M.L., Steen, K.W., Hagen, L., Slupphaug, G. Proteome Science, 9(1): 45 (2011)

8. Photodynamic therapy with hexyl aminolevulinate induces carbonylation, posttranslational modifications and changed expression of proteins in cell survival and cell death pathways. Baglo, Y., Sousa, M.M.L., Slupphaug, G., Hagen, L., Håvåg, S., Helander, L., Zub, K.A., Krokan, H., Gederaas, O.A. Photochemical and Photobiological Sciences, 10:1137-1145 (2011)

7. Divergent β-hairpins determine double-strand versus single-strand substrate recognition of human AlkB-homologues 2 and 3. Monsen, V.T., Sundheim, O., Aas, P.A., Westbyen, M.P., Sousa, M.M.L., Slupphaug, G., Krokan, H.E. Nucleic Acids Research 38(19): 6447-6455 (2010).

6. Identification of a novel, widespread, and functionally important PCNA-binding motif. Gilljam, K.M., Feyzi, E., Aas, P.A., Sousa, M.M.L., Müller, R., Vågbø, C.V., Catterall, T.C., Liabakk, N.B., Slupphaug, G., Drabløs, F., Krokan, H.E., Otterlei, M. The Journal of Cell Biology, 186(5):645–654 (2009).

5. Uracil in DNA and its processing by different DNA glycosylases. Visnes,T., Doseth, B., Pettersen, H.S., Hagen, L., Sousa, M.M.L., Akbari, M., Otterlei, M., Kavli, B., Slupphaug, G. and Krokan, H.E.. Phil. Trans. R. Soc. B 364, 563-568 (2009).

4. Cell cycle-specific UNG2 phosphorylations regulate protein turnover, activity and association with RPA. Hagen, L., Kavli, B.M., Sousa, M.M.L., Torseth, K., Liabakk, N., Sundheim, O., Diaz, J.P., Otterlei, M., Hørning, O., Jensen, O.N., Krokan, H.E., Slupphaug, G. The EMBO Journal 27:51–61 (2008).

3. DNA-uracil and human pathology. Sousa, M.M.L., Krokan, H.E., Slupphaug, G.Molecular Aspects of Medicine 28:276–306 (2007).

2. Human ABH3 structure and key residues for oxidative demethylation to reverse DNA/RNA damage. Sundheim, O., Vågbø, C.B., Bjørås, M., Sousa, M.M.L., Talstad, V., Aas, P.A., Drabløs, F., Krokan, H.E., Tainer, J.A., Slupphaug, G. The EMBO journal, 25(14): 3389–3397 (2006).

1. Sequence similarity-based proteomics in insects: characterization of the larvae venom of the brazilian moth Cerodirphia speciosa. Shevchenko, A., Sousa, M.M.L., Waridel, P., Bittencourt, S.T., Sousa, M.V., and Shevchenko, A. Journal of Proteome Research 4, 862-869 (2005).


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