Ralph Kissen
Om
Arbeidsoppgaver
- bistå i forskningen til faggruppen celle- og molekylærbiologi
- tekniske/administrative oppgaver (romansvarlig, drift av GMO-lab og analyseutstyr, bookitlab, LN2)
- bidra til undervisning i celle- og molekylærbiologi
- opplæring av masterstudenter på utstyr og i teknikker
Kompetanse
- ekstraksjon og analyse av DNA, RNA og proteiner
- ekstraksjon av metabolitter og analyse ved bruk av GC-MS og HPLC
- uttrykk av rekombinante proteiner i Escherichia coli, rensing og funksjonell karakterisering (immunoassay, enzymassay)
- arbeid med planter:
- dyrke in vitro (medium, hydroponi) og på jord
- transformere med Agrobacterium tumefaciens
- genomredigere (CRISPR/Cas9)
- lage, screene og karakterisere mutant-linjer
- forsøk med biotiske og abiotiske stressfaktorer
- arbeid med kiselalger (Phaeodactylum tricornutum):
- dyrke og vedlikeholde kulturer
- transformere med biolistikk og konjugering
- genomredigere (CRISPR/Cas9)
- screene og karakterisere mutant-linjer
Publikasjoner
2021
-
Ahuja, Ishita;
Kissen, Ralph;
Hoang, Linh;
Sporsheim, Bjørnar;
Halle, Kari Krizak;
Wolff, Silje Aase.
(2021)
The imaging of guard cells of thioglucosidase (tgg) mutants of Arabidopsis further links plant chemical defence systems with physical defence barriers.
Cells
Vitenskapelig artikkel
2020
-
Ting, Hieng Ming;
Cheah, Boon Huat;
Chen, Yu-Cheng;
Yeh, Pei-Min;
Cheng, Piu-Ching;
Yeo, Freddy Kuok San.
(2020)
The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses.
Frontiers in Plant Science
Vitenskapelig artikkel
-
Moradi, Babak;
Kissen, Ralph;
Maivan, Hassan Zare;
Hashtroudi, Mehri Seyed;
Sorahinobar, Mona;
Sparstad, Torfinn.
(2020)
Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarker.
Biotechnology Reports
Vitenskapelig artikkel
-
Moradi, Babak;
Kissen, Ralph;
Maivan, Hassan Zare;
Hashtroudi, Mehri Seyed;
Sorahinobar, Mona;
Sparstad, Torfinn.
(2020)
Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination - Polyphenol oxidase (PPOA) as biomarker.
Biotechnology Reports
Vitenskapelig artikkel
2019
-
Sharma, Amit Kumar;
Mühlroth, Alice;
Jouhet, Juliette;
Maréchal, Eric;
Alipanah, Leila;
Kissen, Ralph.
(2019)
The Myb like transcription factor Phosphorus Starvation Response (PtPSR) controls conditional P acquisition and remodeling in marine microalgae.
New Phytologist
Vitenskapelig artikkel
2018
-
Urbancsok, Janos;
Bones, Atle M.;
Kissen, Ralph.
(2018)
Benzyl Cyanide Leads to Auxin-Like Effects Through the Action of Nitrilases in Arabidopsis thaliana.
Frontiers in Plant Science
Vitenskapelig artikkel
-
Urbancsok, Janos;
Bones, Atle M.;
Kissen, Ralph.
(2018)
Arabidopsis mutants impaired in glutathione biosynthesis exhibit higher sensitivity towards the glucosinolate hydrolysis product allyl-isothiocyanate.
Scientific Reports
Vitenskapelig artikkel
-
Ruta, Lavinia L;
Banu, Melania A.;
Neagoe, Aurora D;
Kissen, Ralph;
Bones, Atle M.;
Farcasanu, Ileana C..
(2018)
Accumulation of (Ag(I) by Saccharomyces cereviseae cells expressing plant metallothioneins.
Cells
Vitenskapelig artikkel
2017
-
Urbancsok, Janos;
Bones, Atle M.;
Kissen, Ralph.
(2017)
Glucosinolate-Derived Isothiocyanates Inhibit Arabidopsis Growth and the Potency Depends on their Side Chain Structure.
International Journal of Molecular Sciences
Vitenskapelig artikkel
-
Ruta, Lavinia Liliana;
Lin, Ya-Fen;
Kissen, Ralph;
Nicolau, Ioana;
Neagoe, Aurora Daniela;
Ghenea, Simona.
(2017)
Anchoring plant metallothioneins to the inner face of the plasma membrane of Saccharomyces cerevisiae cells leads to heavy metal accumulation.
PLOS ONE
Vitenskapelig artikkel
-
Ruta, Lavinia Liliana;
Kissen, Ralph;
Nicolau, Ioana;
Neagoe, Aurora Daniela;
Petrescu, Andrei José;
Bones, Atle M..
(2017)
Heavy Metal Accumulation by Saccharomyces cerevisiae Cells Armed with Metal Binding Hexapeptides Targeted to the Inner Face of the Plasma Membrane.
Applied Microbiology and Biotechnology
Vitenskapelig artikkel
2016
-
Kissen, Ralph;
Eberl, Franziska;
Winge, Per;
Uleberg, Eivind;
Martinussen, Inger;
Bones, Atle M..
(2016)
Effect of growth temperature on glucosinolate profiles in Arabidopsis thaliana accessions.
Phytochemistry
Vitenskapelig artikkel
-
Kissen, Ralph;
Øverby, Anders;
Winge, Per;
Bones, Atle M..
(2016)
Allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defence responses in Arabidopsis thaliana.
BMC Genomics
Vitenskapelig artikkel
2015
-
Bones, Atle M.;
Hara, M;
Rossiter, JT;
Kissen, Ralph.
(2015)
Physiology and cellular mechanisms of isothiocyanates and other glucosinolate degradation products in plants.
Frontiers in Plant Science
Leder
2012
-
Kong, Xiang Yi;
Kissen, Ralph;
Bones, Atle M..
(2012)
Characterization of recombinant nitrile-specifier proteins (NSPs) of Arabidopsis thaliana: Dependency on Fe(II) ions and the effect of glucosinolate substrate and reaction conditions.
Phytochemistry
Vitenskapelig artikkel
-
Ahuja, Ishita;
Kissen, Ralph;
Bones, Atle M..
(2012)
Phytoalexins in defense against pathogens.
Trends in Plant Science
Vitenskapelig oversiktsartikkel/review
-
Kissen, Ralph;
Hyldbakk, Elisabeth;
Wang, Chia-Wei Vivian;
Sørmo, Christopher;
Rossiter, John T.;
Bones, Atle M..
(2012)
Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana.
Plant Molecular Biology
Vitenskapelig artikkel
2010
-
Kissen, Ralph;
Winge, Per;
Tran, Diem Hong Thi;
Jørstad, Tommy Stokmo;
Størseth, Trond;
Christensen, Tone.
(2010)
Transcriptional profiling of an Fd-GOGAT1/GLU1 mutant in Arabidopsis thaliana reveals a multiple stress response and extensive reprogramming of the transcriptome.
BMC Genomics
Vitenskapelig artikkel
2009
-
Kissen, Ralph;
Rossiter, JT;
Bones, Atle M..
(2009)
The “mustard oil bomb”: not so easy to assemble?! Localization, expression and distribution of components of the myrosinase enzyme system.
Phytochemistry Reviews
Vitenskapelig artikkel
-
Kissen, Ralph;
Bones, Atle M..
(2009)
Nitrile-specifier proteins involved in glucosinolate hydrolysis in Arabidopsis thaliana.
Journal of Biological Chemistry
Vitenskapelig artikkel
-
Kissen, Ralph;
Pope, Tom W;
Grant, Murray;
Pickett, John A;
Rossiter, John T;
Powell, Glen.
(2009)
Modifying the alkylglucosinolate profile in Arabidopsis thaliana alters the tritrophic interaction with the herbivore Brevicoryne brassicae and parasitoid Diaeretiella rapae.
Journal of Chemical Ecology
Vitenskapelig artikkel
2008
-
Kissen, Ralph;
Bones, Atle M..
(2008)
Characterization of novel nitrile specifier proteins (NSPs) involved in glucosinolate hydrolysis in Arabidopsis thaliana.
Physiologia Plantarum : An International Journal for Plant Biology
Sammendrag/Abstract
Tidsskriftspublikasjoner
-
Ahuja, Ishita;
Kissen, Ralph;
Hoang, Linh;
Sporsheim, Bjørnar;
Halle, Kari Krizak;
Wolff, Silje Aase.
(2021)
The imaging of guard cells of thioglucosidase (tgg) mutants of Arabidopsis further links plant chemical defence systems with physical defence barriers.
Cells
Vitenskapelig artikkel
-
Ting, Hieng Ming;
Cheah, Boon Huat;
Chen, Yu-Cheng;
Yeh, Pei-Min;
Cheng, Piu-Ching;
Yeo, Freddy Kuok San.
(2020)
The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses.
Frontiers in Plant Science
Vitenskapelig artikkel
-
Moradi, Babak;
Kissen, Ralph;
Maivan, Hassan Zare;
Hashtroudi, Mehri Seyed;
Sorahinobar, Mona;
Sparstad, Torfinn.
(2020)
Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarker.
Biotechnology Reports
Vitenskapelig artikkel
-
Moradi, Babak;
Kissen, Ralph;
Maivan, Hassan Zare;
Hashtroudi, Mehri Seyed;
Sorahinobar, Mona;
Sparstad, Torfinn.
(2020)
Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination - Polyphenol oxidase (PPOA) as biomarker.
Biotechnology Reports
Vitenskapelig artikkel
-
Sharma, Amit Kumar;
Mühlroth, Alice;
Jouhet, Juliette;
Maréchal, Eric;
Alipanah, Leila;
Kissen, Ralph.
(2019)
The Myb like transcription factor Phosphorus Starvation Response (PtPSR) controls conditional P acquisition and remodeling in marine microalgae.
New Phytologist
Vitenskapelig artikkel
-
Urbancsok, Janos;
Bones, Atle M.;
Kissen, Ralph.
(2018)
Benzyl Cyanide Leads to Auxin-Like Effects Through the Action of Nitrilases in Arabidopsis thaliana.
Frontiers in Plant Science
Vitenskapelig artikkel
-
Urbancsok, Janos;
Bones, Atle M.;
Kissen, Ralph.
(2018)
Arabidopsis mutants impaired in glutathione biosynthesis exhibit higher sensitivity towards the glucosinolate hydrolysis product allyl-isothiocyanate.
Scientific Reports
Vitenskapelig artikkel
-
Ruta, Lavinia L;
Banu, Melania A.;
Neagoe, Aurora D;
Kissen, Ralph;
Bones, Atle M.;
Farcasanu, Ileana C..
(2018)
Accumulation of (Ag(I) by Saccharomyces cereviseae cells expressing plant metallothioneins.
Cells
Vitenskapelig artikkel
-
Urbancsok, Janos;
Bones, Atle M.;
Kissen, Ralph.
(2017)
Glucosinolate-Derived Isothiocyanates Inhibit Arabidopsis Growth and the Potency Depends on their Side Chain Structure.
International Journal of Molecular Sciences
Vitenskapelig artikkel
-
Ruta, Lavinia Liliana;
Lin, Ya-Fen;
Kissen, Ralph;
Nicolau, Ioana;
Neagoe, Aurora Daniela;
Ghenea, Simona.
(2017)
Anchoring plant metallothioneins to the inner face of the plasma membrane of Saccharomyces cerevisiae cells leads to heavy metal accumulation.
PLOS ONE
Vitenskapelig artikkel
-
Ruta, Lavinia Liliana;
Kissen, Ralph;
Nicolau, Ioana;
Neagoe, Aurora Daniela;
Petrescu, Andrei José;
Bones, Atle M..
(2017)
Heavy Metal Accumulation by Saccharomyces cerevisiae Cells Armed with Metal Binding Hexapeptides Targeted to the Inner Face of the Plasma Membrane.
Applied Microbiology and Biotechnology
Vitenskapelig artikkel
-
Kissen, Ralph;
Eberl, Franziska;
Winge, Per;
Uleberg, Eivind;
Martinussen, Inger;
Bones, Atle M..
(2016)
Effect of growth temperature on glucosinolate profiles in Arabidopsis thaliana accessions.
Phytochemistry
Vitenskapelig artikkel
-
Kissen, Ralph;
Øverby, Anders;
Winge, Per;
Bones, Atle M..
(2016)
Allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defence responses in Arabidopsis thaliana.
BMC Genomics
Vitenskapelig artikkel
-
Bones, Atle M.;
Hara, M;
Rossiter, JT;
Kissen, Ralph.
(2015)
Physiology and cellular mechanisms of isothiocyanates and other glucosinolate degradation products in plants.
Frontiers in Plant Science
Leder
-
Kong, Xiang Yi;
Kissen, Ralph;
Bones, Atle M..
(2012)
Characterization of recombinant nitrile-specifier proteins (NSPs) of Arabidopsis thaliana: Dependency on Fe(II) ions and the effect of glucosinolate substrate and reaction conditions.
Phytochemistry
Vitenskapelig artikkel
-
Ahuja, Ishita;
Kissen, Ralph;
Bones, Atle M..
(2012)
Phytoalexins in defense against pathogens.
Trends in Plant Science
Vitenskapelig oversiktsartikkel/review
-
Kissen, Ralph;
Hyldbakk, Elisabeth;
Wang, Chia-Wei Vivian;
Sørmo, Christopher;
Rossiter, John T.;
Bones, Atle M..
(2012)
Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana.
Plant Molecular Biology
Vitenskapelig artikkel
-
Kissen, Ralph;
Winge, Per;
Tran, Diem Hong Thi;
Jørstad, Tommy Stokmo;
Størseth, Trond;
Christensen, Tone.
(2010)
Transcriptional profiling of an Fd-GOGAT1/GLU1 mutant in Arabidopsis thaliana reveals a multiple stress response and extensive reprogramming of the transcriptome.
BMC Genomics
Vitenskapelig artikkel
-
Kissen, Ralph;
Rossiter, JT;
Bones, Atle M..
(2009)
The “mustard oil bomb”: not so easy to assemble?! Localization, expression and distribution of components of the myrosinase enzyme system.
Phytochemistry Reviews
Vitenskapelig artikkel
-
Kissen, Ralph;
Bones, Atle M..
(2009)
Nitrile-specifier proteins involved in glucosinolate hydrolysis in Arabidopsis thaliana.
Journal of Biological Chemistry
Vitenskapelig artikkel
-
Kissen, Ralph;
Pope, Tom W;
Grant, Murray;
Pickett, John A;
Rossiter, John T;
Powell, Glen.
(2009)
Modifying the alkylglucosinolate profile in Arabidopsis thaliana alters the tritrophic interaction with the herbivore Brevicoryne brassicae and parasitoid Diaeretiella rapae.
Journal of Chemical Ecology
Vitenskapelig artikkel
-
Kissen, Ralph;
Bones, Atle M..
(2008)
Characterization of novel nitrile specifier proteins (NSPs) involved in glucosinolate hydrolysis in Arabidopsis thaliana.
Physiologia Plantarum : An International Journal for Plant Biology
Sammendrag/Abstract