Ralph Kissen
Om
Arbeidsoppgaver
- bistå i forskningen til faggruppen celle- og molekylærbiologi
- tekniske/administrative oppgaver (romansvarlig, drift av GMO-lab og analyseutstyr, bookitlab, LN2)
- bidra til undervisning i celle- og molekylærbiologi
- opplæring av masterstudenter på utstyr og i teknikker
Kompetanse
- ekstraksjon og analyse av DNA, RNA og proteiner
- ekstraksjon av metabolitter og analyse ved bruk av GC-MS og HPLC
- uttrykk av rekombinante proteiner i Escherichia coli, rensing og funksjonell karakterisering (immunoassay, enzymassay)
- arbeid med planter:
- dyrke in vitro (medium, hydroponi) og på jord
- transformere med Agrobacterium tumefaciens
- genomredigere (CRISPR/Cas9)
- lage, screene og karakterisere mutant-linjer
- forsøk med biotiske og abiotiske stressfaktorer
- arbeid med kiselalger (Phaeodactylum tricornutum):
- dyrke og vedlikeholde kulturer
- transformere med biolistikk og konjugering
- genomredigere (CRISPR/Cas9)
- screene og karakterisere mutant-linjer
Publikasjoner
2021
-
Ahuja, Ishita;
Kissen, Ralph;
Hoang, Linh;
Sporsheim, Bjørnar;
Halle, Kari Krizak;
Wolff, Silje Aase.
(2021)
The imaging of guard cells of thioglucosidase (tgg) mutants of Arabidopsis further links plant chemical defence systems with physical defence barriers.
Cells
Vitenskapelig artikkel
2020
-
Ting, Hieng Ming;
Cheah, Boon Huat;
Chen, Yu-Cheng;
Yeh, Pei-Min;
Cheng, Piu-Ching;
Yeo, Freddy Kuok San.
(2020)
The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses.
Frontiers in Plant Science
Vitenskapelig artikkel
-
Moradi, Babak;
Kissen, Ralph;
Maivan, Hassan Zare;
Hashtroudi, Mehri Seyed;
Sorahinobar, Mona;
Sparstad, Torfinn.
(2020)
Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarker.
Biotechnology Reports
Vitenskapelig artikkel
-
Moradi, Babak;
Kissen, Ralph;
Maivan, Hassan Zare;
Hashtroudi, Mehri Seyed;
Sorahinobar, Mona;
Sparstad, Torfinn.
(2020)
Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination - Polyphenol oxidase (PPOA) as biomarker.
Biotechnology Reports
Vitenskapelig artikkel
2019
-
Sharma, Amit Kumar;
Mühlroth, Alice;
Jouhet, Juliette;
Maréchal, Eric;
Alipanah, Leila;
Kissen, Ralph.
(2019)
The Myb like transcription factor Phosphorus Starvation Response (PtPSR) controls conditional P acquisition and remodeling in marine microalgae.
New Phytologist
Vitenskapelig artikkel
2018
-
Ruta, Lavinia L;
Banu, Melania A.;
Neagoe, Aurora D;
Kissen, Ralph;
Bones, Atle M.;
Farcasanu, Ileana C..
(2018)
Accumulation of (Ag(I) by Saccharomyces cereviseae cells expressing plant metallothioneins.
Cells
Vitenskapelig artikkel
-
Urbancsok, Janos;
Bones, Atle M.;
Kissen, Ralph.
(2018)
Benzyl Cyanide Leads to Auxin-Like Effects Through the Action of Nitrilases in Arabidopsis thaliana.
Frontiers in Plant Science
Vitenskapelig artikkel
-
Urbancsok, Janos;
Bones, Atle M.;
Kissen, Ralph.
(2018)
Arabidopsis mutants impaired in glutathione biosynthesis exhibit higher sensitivity towards the glucosinolate hydrolysis product allyl-isothiocyanate.
Scientific Reports
Vitenskapelig artikkel
2017
-
Ruta, Lavinia Liliana;
Lin, Ya-Fen;
Kissen, Ralph;
Nicolau, Ioana;
Neagoe, Aurora Daniela;
Ghenea, Simona.
(2017)
Anchoring plant metallothioneins to the inner face of the plasma membrane of Saccharomyces cerevisiae cells leads to heavy metal accumulation.
PLOS ONE
Vitenskapelig artikkel
-
Ruta, Lavinia Liliana;
Kissen, Ralph;
Nicolau, Ioana;
Neagoe, Aurora Daniela;
Petrescu, Andrei José;
Bones, Atle M..
(2017)
Heavy Metal Accumulation by Saccharomyces cerevisiae Cells Armed with Metal Binding Hexapeptides Targeted to the Inner Face of the Plasma Membrane
.
Applied Microbiology and Biotechnology
Vitenskapelig artikkel
-
Urbancsok, Janos;
Bones, Atle M.;
Kissen, Ralph.
(2017)
Glucosinolate-Derived Isothiocyanates Inhibit Arabidopsis Growth and the Potency Depends on their Side Chain Structure
.
International Journal of Molecular Sciences
Vitenskapelig artikkel
2016
-
Kissen, Ralph;
Eberl, Franziska;
Winge, Per;
Uleberg, Eivind;
Martinussen, Inger;
Bones, Atle M..
(2016)
Effect of growth temperature on glucosinolate profiles in Arabidopsis thaliana accessions.
Phytochemistry
Vitenskapelig artikkel
-
Kissen, Ralph;
Øverby, Anders;
Winge, Per;
Bones, Atle M..
(2016)
Allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defence responses in Arabidopsis thaliana
.
BMC Genomics
Vitenskapelig artikkel
2015
-
Bones, Atle M.;
Hara, M;
Rossiter, JT;
Kissen, Ralph.
(2015)
Physiology and cellular mechanisms of isothiocyanates and other glucosinolate degradation products in plants.
Frontiers in Plant Science
Leder
2012
-
Ahuja, Ishita;
Kissen, Ralph;
Bones, Atle M..
(2012)
Phytoalexins in defense against pathogens.
Trends in Plant Science
Vitenskapelig oversiktsartikkel/review
-
Kissen, Ralph;
Hyldbakk, Elisabeth;
Wang, Chia-Wei Vivian;
Sørmo, Christopher;
Rossiter, John T.;
Bones, Atle M..
(2012)
Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana.
Plant Molecular Biology
Vitenskapelig artikkel
-
Kong, Xiang Yi;
Kissen, Ralph;
Bones, Atle M..
(2012)
Characterization of recombinant nitrile-specifier proteins (NSPs) of Arabidopsis thaliana: Dependency on Fe(II) ions and the effect of glucosinolate substrate and reaction conditions.
Phytochemistry
Vitenskapelig artikkel
2010
-
Kissen, Ralph;
Winge, Per;
Tran, Diem Hong Thi;
Jørstad, Tommy Stokmo;
Størseth, Trond;
Christensen, Tone.
(2010)
Transcriptional profiling of an Fd-GOGAT1/GLU1 mutant in Arabidopsis thaliana reveals a multiple stress response and extensive reprogramming of the transcriptome.
BMC Genomics
Vitenskapelig artikkel
2009
-
Kissen, Ralph;
Pope, Tom W;
Grant, Murray;
Pickett, John A;
Rossiter, John T;
Powell, Glen.
(2009)
Modifying the alkylglucosinolate profile in Arabidopsis thaliana alters the tritrophic interaction with the herbivore Brevicoryne brassicae and parasitoid Diaeretiella rapae.
Journal of Chemical Ecology
Vitenskapelig artikkel
-
Kissen, Ralph;
Bones, Atle M..
(2009)
Nitrile-specifier proteins involved in glucosinolate hydrolysis in Arabidopsis thaliana.
Journal of Biological Chemistry
Vitenskapelig artikkel
-
Kissen, Ralph;
Rossiter, JT;
Bones, Atle M..
(2009)
The “mustard oil bomb”: not so easy to assemble?! Localization, expression and distribution of components of the myrosinase enzyme system.
Phytochemistry Reviews
Vitenskapelig artikkel
2008
-
Kissen, Ralph;
Bones, Atle M..
(2008)
Characterization of novel nitrile specifier proteins (NSPs) involved in glucosinolate hydrolysis in Arabidopsis thaliana.
Physiologia Plantarum : An International Journal for Plant Biology
Sammendrag/abstract
Tidsskriftspublikasjoner
-
Ahuja, Ishita;
Kissen, Ralph;
Hoang, Linh;
Sporsheim, Bjørnar;
Halle, Kari Krizak;
Wolff, Silje Aase.
(2021)
The imaging of guard cells of thioglucosidase (tgg) mutants of Arabidopsis further links plant chemical defence systems with physical defence barriers.
Cells
Vitenskapelig artikkel
-
Ting, Hieng Ming;
Cheah, Boon Huat;
Chen, Yu-Cheng;
Yeh, Pei-Min;
Cheng, Piu-Ching;
Yeo, Freddy Kuok San.
(2020)
The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses.
Frontiers in Plant Science
Vitenskapelig artikkel
-
Moradi, Babak;
Kissen, Ralph;
Maivan, Hassan Zare;
Hashtroudi, Mehri Seyed;
Sorahinobar, Mona;
Sparstad, Torfinn.
(2020)
Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarker.
Biotechnology Reports
Vitenskapelig artikkel
-
Moradi, Babak;
Kissen, Ralph;
Maivan, Hassan Zare;
Hashtroudi, Mehri Seyed;
Sorahinobar, Mona;
Sparstad, Torfinn.
(2020)
Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination - Polyphenol oxidase (PPOA) as biomarker.
Biotechnology Reports
Vitenskapelig artikkel
-
Sharma, Amit Kumar;
Mühlroth, Alice;
Jouhet, Juliette;
Maréchal, Eric;
Alipanah, Leila;
Kissen, Ralph.
(2019)
The Myb like transcription factor Phosphorus Starvation Response (PtPSR) controls conditional P acquisition and remodeling in marine microalgae.
New Phytologist
Vitenskapelig artikkel
-
Ruta, Lavinia L;
Banu, Melania A.;
Neagoe, Aurora D;
Kissen, Ralph;
Bones, Atle M.;
Farcasanu, Ileana C..
(2018)
Accumulation of (Ag(I) by Saccharomyces cereviseae cells expressing plant metallothioneins.
Cells
Vitenskapelig artikkel
-
Urbancsok, Janos;
Bones, Atle M.;
Kissen, Ralph.
(2018)
Benzyl Cyanide Leads to Auxin-Like Effects Through the Action of Nitrilases in Arabidopsis thaliana.
Frontiers in Plant Science
Vitenskapelig artikkel
-
Urbancsok, Janos;
Bones, Atle M.;
Kissen, Ralph.
(2018)
Arabidopsis mutants impaired in glutathione biosynthesis exhibit higher sensitivity towards the glucosinolate hydrolysis product allyl-isothiocyanate.
Scientific Reports
Vitenskapelig artikkel
-
Ruta, Lavinia Liliana;
Lin, Ya-Fen;
Kissen, Ralph;
Nicolau, Ioana;
Neagoe, Aurora Daniela;
Ghenea, Simona.
(2017)
Anchoring plant metallothioneins to the inner face of the plasma membrane of Saccharomyces cerevisiae cells leads to heavy metal accumulation.
PLOS ONE
Vitenskapelig artikkel
-
Ruta, Lavinia Liliana;
Kissen, Ralph;
Nicolau, Ioana;
Neagoe, Aurora Daniela;
Petrescu, Andrei José;
Bones, Atle M..
(2017)
Heavy Metal Accumulation by Saccharomyces cerevisiae Cells Armed with Metal Binding Hexapeptides Targeted to the Inner Face of the Plasma Membrane
.
Applied Microbiology and Biotechnology
Vitenskapelig artikkel
-
Urbancsok, Janos;
Bones, Atle M.;
Kissen, Ralph.
(2017)
Glucosinolate-Derived Isothiocyanates Inhibit Arabidopsis Growth and the Potency Depends on their Side Chain Structure
.
International Journal of Molecular Sciences
Vitenskapelig artikkel
-
Kissen, Ralph;
Eberl, Franziska;
Winge, Per;
Uleberg, Eivind;
Martinussen, Inger;
Bones, Atle M..
(2016)
Effect of growth temperature on glucosinolate profiles in Arabidopsis thaliana accessions.
Phytochemistry
Vitenskapelig artikkel
-
Kissen, Ralph;
Øverby, Anders;
Winge, Per;
Bones, Atle M..
(2016)
Allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defence responses in Arabidopsis thaliana
.
BMC Genomics
Vitenskapelig artikkel
-
Bones, Atle M.;
Hara, M;
Rossiter, JT;
Kissen, Ralph.
(2015)
Physiology and cellular mechanisms of isothiocyanates and other glucosinolate degradation products in plants.
Frontiers in Plant Science
Leder
-
Ahuja, Ishita;
Kissen, Ralph;
Bones, Atle M..
(2012)
Phytoalexins in defense against pathogens.
Trends in Plant Science
Vitenskapelig oversiktsartikkel/review
-
Kissen, Ralph;
Hyldbakk, Elisabeth;
Wang, Chia-Wei Vivian;
Sørmo, Christopher;
Rossiter, John T.;
Bones, Atle M..
(2012)
Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana.
Plant Molecular Biology
Vitenskapelig artikkel
-
Kong, Xiang Yi;
Kissen, Ralph;
Bones, Atle M..
(2012)
Characterization of recombinant nitrile-specifier proteins (NSPs) of Arabidopsis thaliana: Dependency on Fe(II) ions and the effect of glucosinolate substrate and reaction conditions.
Phytochemistry
Vitenskapelig artikkel
-
Kissen, Ralph;
Winge, Per;
Tran, Diem Hong Thi;
Jørstad, Tommy Stokmo;
Størseth, Trond;
Christensen, Tone.
(2010)
Transcriptional profiling of an Fd-GOGAT1/GLU1 mutant in Arabidopsis thaliana reveals a multiple stress response and extensive reprogramming of the transcriptome.
BMC Genomics
Vitenskapelig artikkel
-
Kissen, Ralph;
Pope, Tom W;
Grant, Murray;
Pickett, John A;
Rossiter, John T;
Powell, Glen.
(2009)
Modifying the alkylglucosinolate profile in Arabidopsis thaliana alters the tritrophic interaction with the herbivore Brevicoryne brassicae and parasitoid Diaeretiella rapae.
Journal of Chemical Ecology
Vitenskapelig artikkel
-
Kissen, Ralph;
Bones, Atle M..
(2009)
Nitrile-specifier proteins involved in glucosinolate hydrolysis in Arabidopsis thaliana.
Journal of Biological Chemistry
Vitenskapelig artikkel
-
Kissen, Ralph;
Rossiter, JT;
Bones, Atle M..
(2009)
The “mustard oil bomb”: not so easy to assemble?! Localization, expression and distribution of components of the myrosinase enzyme system.
Phytochemistry Reviews
Vitenskapelig artikkel
-
Kissen, Ralph;
Bones, Atle M..
(2008)
Characterization of novel nitrile specifier proteins (NSPs) involved in glucosinolate hydrolysis in Arabidopsis thaliana.
Physiologia Plantarum : An International Journal for Plant Biology
Sammendrag/abstract